AB747

Anti-Collagen Type III Antibody

Chemicon^®, from rabbit

• Synonym(s): Anti-EDS4A, Anti-EDSVASC, Anti-PMGEDSV
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: fractionated antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunofluorescence: suitable
• suitability: not suitable for Western blot; not suitable for immunohistochemistry (Paraffin)
• NCBI accession no.: NM_000090.3
• UniProt accession no.: P02461
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... COL3A1(1281)

Description

Specificity

The antibody reacts with native and heat denatured (non-reduced) human collagen type III. Cross reactions with other types of collagen under native conditions do occur. There is 10% cross reactivity with human collagen type I, 2% cross reactivity with human collagen type II, and 4% cross reactivity with human collagen type IV and V. There is no cross reactivity with other human plasma proteins under native ELISA conditions. Reactivity under denatured conditions has not been examined.



Cross Reactivity Percent

Human collagen type I 10%

Human collagen type II 2%

Human collagen type III 100%

Human collagen type IV and V 4%



Human plasma proteins do not interfere with binding to collagen.

Immunogen

Human placental collagen type III

Application

ELISA 1:3,000

Indirect immunofluorescence on cryostat sections or cultured cells 1:20-1:40

Optimal working dilutions must be determined by the end user.

This Anti-Collagen Type III Antibody is validated for use in ELISA, IF for the detection of Collagen Type III.

Physical form

500ul containing of 0.15M NaCl, 10 mM sodium phosphate pH 7.5, with 0.1% mannitol and o.1% dextran as stabilizers contains no preservatives.

Immunoglobulin fraction was prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiserum cross-absorbed over immobilized human serum proteins, immunoglobulins, and collagen types I, II, IV and V.

Format: Purified

Storage and Stability

Antibody is stable for 12 months from date of receipt when stored at 2-8ºC. Remove any nondispersed aggregates by microcentrifugation prior to use.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable