524390

Phorbol-12,13-dibutyrate

Strong irritant for mouse skin, but only moderately active as a tumor promoter.

• Synonym(s): Phorbol-12,13-dibutyrate, PDBu, PKC Activator II
• Empirical Formula (Hill Notation): C₂₈H₄₀O₈
• CAS Number: 37558-16-0
• Molecular Weight: 504.61
• MDL number: MFCD00036783
• UNSPSC Code: 12352211

Properties

• Quality Level: 100
• Assay: ≥99% (HPLC)
• form: solid (crystalline)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: clear
• solubility: DMSO: 10 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C28H40O8/c1-7-9-20(30)35-24-16(4)27(34)18(22-25(5,6)28(22,24)36-21(31)10-8-2)12-17(14-29)13-26(33)19(27)11-15(3)23(26)32/h11-12,16,18-19,22,24,29,33-34H,7-10,13-14H2,1-6H3/t16-,18+,19-,22-,24-,26-,27-,28-/m1/s1
• InChI key: BQJRUJTZSGYBEZ-YVQNUNKESA-N

Description

General description

Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C (PKC). Stimulates the phosphorylation of Na⁺-K⁺ ATPase, thereby inhibiting its activity. Promotes the expression of iNOS in cultured hepatocytes. PDBu can be diluted to 30 µM in aqueous solutions, so it is commonly used in binding studies or in applications requiring high concentrations. It is easier to remove PDBu than PMA from cells in culture.

Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C. Stimulates the phosphorylation of Na⁺,K⁺- ATPase, thereby inhibiting its activity. Promotes the expression of inducible NOS in cultured hepatocytes. Commonly used in binding studies or in applications requiring high concentrations of phorbol compounds.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Na⁺,K⁺- ATPase

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Preparation Note

Further dilute with aqueous buffers just prior to use.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Fisone, G., et al. 1995. J. Biol. Chem. 270, 2427.
Lee, E.J., et al. 1994. Brain Res. 665, 127.
Critz, S.D., et al. 1993. J. Neurochem.60, 1175.
Iredale, P.A., et al. 1993. Biochem. Pharmacol.45, 611.
Critz, S.D., and Byrne J.H. 1992. J. Neurophys.68, 1079.
Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
Ward C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS05,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H300 + H310 + H330 - H314 - H317 - H334 - H351
• Precautionary Statements: P202 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable