Skip to Content
Merck
All Photos(1)

Key Documents

516485

Sigma-Aldrich

Pepstatin A Methyl Ester

A cell-permeable methyl ester derivative of Pepstatin A that acts as a potent, non-competitive, transition-state analog inhibitor of γ-secretase.

Synonym(s):

Pepstatin A Methyl Ester, Isovaleryl-V V-Sta-A-Sta-OCH₃, PME

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C35H65N5O9
Molecular Weight:
699.92
UNSPSC Code:
12352108
NACRES:
NA.77

Quality Level

Assay

≥95% (HPLC)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

DMSO: 5 mg/mL

shipped in

wet ice

storage temp.

−20°C

General description

A cell-permeable methyl ester derivative of Pepstatin A (Cat. No. 516481) that acts as a potent, non-competitive, transition-state analog inhibitor of γ-secretase (Kis = 150 nM, Kii = 320 nM in solubilized human γ-secretase at 20°C; Kis is the inhibition constant for inhibitor binding to the free enzyme and Kii is the inhibition constant for inhibitor binding to the Enzyme-Substrate complex). Sta = (3S,4S)-4-Amino-3-hydroxy-6-methyl-heptanoic acid.
A cell-permeable methyl ester derivative of Pepstatin A (Cat. No. 516482) that acts as a potent, non-competitive, transition-state analog inhibitor of γ-secretase (Kis = 15 nM and Kii = 32 nM in solubilized human γ-secretase; Kis is the inhibition constant for inhibitor binding to the free enzyme and Kii is the inhibition constant for inhibitor binding to the Enzyme-Substrate complex).

Biochem/physiol Actions

Cell permeable: yes
Primary Target
γ-secretase
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.

Other Notes

Tian, G., et al. 2002. J. Biol. Chem.277, 31499.
Zhang, L., et al. 2001. Biochemistry40, 5049.
Murphy, M.P., et al. 1999. J. Biol. Chem.274, 11914.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service