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05-633

Sigma-Aldrich

Anti-BrdU Antibody, clone BU-1

culture supernatant, clone BU-1, Upstate®

Synonym(s):

Anti-BrdU antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

BU-1, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable

isotype

IgG2a

shipped in

wet ice

target post-translational modification

unmodified

General description

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Specificity

5-Bromo-2-deoxyuridine

Immunogen

5-iodouridine:ovalbumin conjugate

Application

Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC.

Quality

A representative lot was evaluated by immunocytochemistry on HeLa cells. A 1:2-1:4 dilution from a representative lot showed positive immunostaining for Anti-BrdU in HeLa cells fixed with 95% ethanol, 5% glacial acetic acid. This lot was tested using the DNA Replication Assay Kit (Catalog # 17-316).

Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.

Target description

none given

Physical form

4 vials, each vial containing mouse culture supernatant lyophilized from 2.5 mL. The supernatant contains mycoplasma, which contribute enzymes that facilitate antibody binding. Lyophilized powder.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Immunofluorescence labeling indices in myeloma and related monoclonal gammopathies.
Greipp, P R, et al.
Mayo Clinic Proceedings. Mayo Clinic, 62, 969-977 (1987)
X Gao et al.
Cell death & disease, 6, e1610-e1610 (2015-01-23)
G-quadruplex (G4) DNA and G4 DNA resolvase are involved in a variety of biological processes. To understand the biological function of G4 DNA structures and their resolvases in spermatogenesis, we investigated the distribution of G4 structures in mouse testis and
Models of S-phase determination in lymphomas from flow cytometric DNA content histograms: comparison with the bromodeoxyuridine labeling index.
Beauregard, P, et al.
Hematologic Pathology, 5, 177-183 (1991)
Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
Tudor, CS; Bruns, H; Daniel, C; Distel, LV; Hartmann, A; Gerbitz, A; Buettner, MJ
Testing null
Difference between random and imprinted X inactivation in common voles.
Elena V Dementyeva,Alexander I Shevchenko,Olga V Anopriyenko,Nina A Mazurok et al.
Chromosoma null

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