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Key Documents

05-503-I

Sigma-Aldrich

Anti-LacI Antibody, clone 9A5

clone 9A5, from mouse

Synonym(s):

Lactose operon repressor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9A5, monoclonal

species reactivity

E. coli

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_414879

UniProt accession no.

P03023

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

Escherichia coli K12 ... Laci(945007)

General description

The lactose operon repressor binds to a regulatory element, an operator gene, to form a genetic switch, the lac operon. This switch negatively regulates the expression of structural genes that follow the site of the operator gene. Inducers may bind to the repressor to reduce or eliminate the affinity of the repressor for its operator, and thereby allow for transcription of respective structural genes. Conversely, other effector molecules may increase the binding affinity of the repressor for its operator and thereby inhibit gene transcription. The inducer or effector molecules that associate with a repressor serve as a chemical signal, which monitors the metabolic state of protein(s) expressed by their respective genes.

Immunogen

TrpE fused with full-length E. Coli LacI.

Application

Anti-LacI Antibody, clone 9A5 is an antibody against LacI for use in Western Blotting.
Research Category
Secondary & Control Antibodies
Research Sub Category
Epitope Tags

Quality

Evaluated by Western Blot in Rosetta BL21 (DE3) E. Coli cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected LacI in 10 µg of Rosetta BL21 (DE3) E. Coli cell lysate.

Target description

~38 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide (This antibody is purified from supernatent.).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rosetta BL21 (DE3) competent E. Coli cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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