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05-440

Sigma-Aldrich

Anti-Cbl Antibody, clone 7G10

clone 7G10, Upstate®, from mouse

Synonym(s):

Cas-Br-M (murine) ecotropic retroviral transforming sequence, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-CBL, RING finger protein 55, Signal transduction protein CBL, oncogene CBL2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7G10, monoclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_005188

UniProt accession no.

P22681

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CBL(867)

General description

Casitas B-lineage lymphoma (Cbl), is an oncogene which causes pre B-cell lymphomas. It participates in the regulation of cell signaling and has the ability to interact with many proteins such as receptor/non-receptor PTK′s, structural proteins, and novel proteins that are not well understood. Diseases associated with Cbl are autoimmune diseases, diabetes, and leukemia. By modifying Cbl′s ability to bind to oncogenic proteins, Cbl may be developed into an anti-cancer tool and in the near future used in therapeutic intervention.

Specificity

Antibody recognizes amino acids 878-906 on human Cbl

Immunogen

Epitope: a.a. 878-906
GST fusion protein corresponding to residues 878-906 of human Cbl

Application

Immunocytochemistry:
5-10 μg/mL of a previous lot showed positive immunostaining for Cbl in 3T3/A31 cells fixed with ice cold Methanol/Ethanol [1:1] for 1 minute.

Immunoprecipitation:
2 μg of a previous lot immunoprecipitated Cbl from 1 mg of a mouse 3T3/A31 cell RIPA lysate, as reported by an independent laboratory.
Research Category
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
This Anti-Cbl Antibody, clone 7G10 is validated for use in IP, WB, IC for the detection of Cbl.

Quality

routinely evaluated by immunoblot on RIPA lysates from human Jurkat, mouse 3T3/A31, TF-1, or FDC-P1/Mac11

Target description

120kDa

Physical form

Format: Purified
Protein G Chromatography
Purified IgG1k in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cbl-b is a negative regulator of inflammatory cytokines produced by IgE-activated mast cells.
Sonja E Gustin, Christine B F Thien, Wallace Y Langdon
Journal of immunology (Baltimore, Md. : 1950) (1950)
T J Blake et al.
Oncogene, 7(4), 757-762 (1992-04-01)
The murine Cas NS-1 retrovirus carries the v-cbl oncogene and induces pro-B, pre-B and myeloid tumors in mice inoculated at birth. The human homolog of v-cbl is located on chromosome 11q23.3, which is in the region of translocation breakpoints in
Hua-Bei Guo et al.
Glycobiology, 19(5), 547-559 (2009-02-20)
Changes in the expression of N-glycan branching glycosyltransferases can alter cell surface receptor functions, involving their levels of cell surface retention, rates of internalization into the endosomal compartment, and subsequent intracellular signaling. To study in detail the regulation of signaling
Sabine A Bisson et al.
Oncogene, 21(23), 3677-3687 (2002-05-29)
The c-Cbl proto-oncogene acts as an E3 ubiquitin ligase via its RING finger domain to negatively regulate activated cellular signal transduction pathways. We have identified an aberrant Cbl-protein of approximately 95 kDa, which we have called p95Cbl, from the murine
Karisa C Schreck et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 27(22), 6197-6208 (2021-08-27)
Selective RAF-targeted therapy is effective in some patients with BRAFV600E-mutated glioma, though emergent and adaptive resistance occurs through ill-defined mechanisms. Paired pre-/post- RAF inhibitor (RAFi)-treated glioma samples (N = 15) were obtained and queried for treatment-emergent genomic alterations using DNA
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