05-1000
Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)
clone 4A4, Upstate®, from mouse
Synonym(s):
Clone 4A4 Anti-Phosphoserine, Phosphoserine Detection Antibody
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All Photos(9)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
4A4, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
shipped in
dry ice
target post-translational modification
phosphorylation (pSer)
General description
The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (>95%).
Specificity
This antibody recognizes serine-phosphorylated proteins from all species.
Immunogen
Phosphoserine coupled to KLH.
Application
Detect Phosphoserine using this Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) validated for use in ELISA, FC, IF, IH(P) & WB.
Immunofluorescence
Flow Cytometry
Immunohistochemistry (Paraffin)
ELISA
Flow Cytometry
Immunohistochemistry (Paraffin)
ELISA
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Quality
Routinely evaluated by Western Blot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
Western Blot Analysis:
0.5–2 μg/mL of this lot detected serinephosphorylated proteins in a lysate from either insulin or Calyculin A/Okadaic-treated human A431 carcinoma cells.
Western Blot Analysis:
0.5–2 μg/mL of this lot detected serinephosphorylated proteins in a lysate from either insulin or Calyculin A/Okadaic-treated human A431 carcinoma cells.
Target description
Dependent upon the molecular weight of the serine phosphorylated protein being detected.
Physical form
Format: Purified
Protein G-Sepharose Chromatography
Purified mouse monoclonal IgG1 in buffer containing PBS with 0.1% sodium azide and 30% glycerol.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Nucleocytoplasmic shuttling of a GATA transcription factor functions as a development timer.
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It is well established that the tumorigenic potential of nucleophosmin (NPM)-anaplastic lymphoma kinase (ALK), an oncogenic tyrosine kinase, is dependent on its tyrosine phosphorylation. Using tandem affinity purification-mass spectrometry, we found evidence of phosphorylation of three serine residues of NPM-ALK
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C Z Cotrim et al.
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Two thirds of breast cancers express estrogen receptors (ER). ER alpha (ERα) mediates breast cancer cell proliferation, and expression of ERα is the standard choice to indicate adjuvant endocrine therapy. ERbeta (ERβ) inhibits growth in vitro; its effects in vivo
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Cole JA, Meyers SA
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