HPLC Analysis of Xylene Isomers on Discovery^® Zr-Carbon

column

Discovery Zr-Carbon, 15 cm × 4.6 mm I.D., 3 μm particles (65730-U)

mobile phase

[A] water: [B] acetonitrile; (50:50, A:B)

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 254 nm

injection

1 μL

sample

o-xylene, m-xylene, p-xylene (290 μg/mL)