Documentos clave
MSP07
Membrane Scaffold Protein 1E2D1 D73C
recombinant, expressed in E. coli, Cysteine substituted at position 73
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About This Item
Productos recomendados
recombinant
expressed in E. coli
assay
≥90% (SDS-GE)
form
buffered aqueous solution
shipped in
ambient
storage temp.
−20°C
General description
The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E2D1 D73C variant of MSP1 differs from MSP1 in the following facets:
- It deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence3 (which is known separately as MSP1D1).
- It repeats the Helix 4 (H4) and Helix 5 (H5) sequences of the original MSP1 sequence between the parent Helix 5 (H5) and Helix 6 (H6) segments of MSP1 1D1.
- It substitutes a cysteine (C) residue for an aspartic acid (D) residue in the Helix 2 (H2) portion of the protein, at position 73 of the original native A-1 sequence.
Application
Legal Information
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocolos
Protocols for Membrane Scaffold Proteins and Nanodisc Formation
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