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Key Documents

H9268

Sigma-Aldrich

Hexadimethrine bromide

≥94% (titration)

Synonym(s):

1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide, Polybrene

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
41106502
NACRES:
NA.26

Quality Level

assay

≥94% (titration)

storage temp.

2-8°C

InChI

1S/C10H24N2.C3H6Br2/c1-11(2)9-7-5-6-8-10-12(3)4;4-2-1-3-5/h5-10H2,1-4H3;1-3H2

InChI key

KZKAYEGOIJEWQB-UHFFFAOYSA-N

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General description

Hexadimethrine bromide is a quaternary ammonium compound which works as a heparin antagonist. It is also involved in the transformation of low molecular weight DNA.

Application

Hexadimethrine bromide can be used to transfect mammalian cells with DNA. It can be used to increase the efficiency of lipofection transfections.
Hexadimethrine bromide has been used for lentivirus infection in cells. It has been used for infection of cells with retroviral supernatant.

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Description
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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Redei GP
Encyclopedia of Genetics, Genomics, Proteomics, and Informatics (2008)
S Kawai et al.
Molecular and cellular biology, 4(6), 1172-1174 (1984-06-01)
A new procedure for DNA transfection has been developed in a system of chicken embryo fibroblast cells and cloned Rous sarcoma virus DNA by using a polycation reagent as a mediator to adsorb DNA to the cell surface and dimethyl
E2F1 modulates p38 MAPK phosphorylation via transcriptional regulation of ASK1 and Wip1.
Hershko T
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Molecular pathways regulating pro-migratory effects of Hedgehog signaling.
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Cutaneous melanoma represents the most fatal skin cancer due to its high metastatic capacity. According to the "phenotype switching" model, the aggressive nature of melanoma cells results from their intrinsic potential to dynamically switch from a high-proliferative/low-invasive to a low-proliferative/high-invasive

Articles

The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Protocols

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recording of fluorescent signals from individual cells.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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