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Merck
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HSTCMAG-28SK

Millipore

MILLIPLEX® Human High Sensitivity T Cell Panel - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

Sinonimo/i:

Human high-sensitivity cytokine multiplex kit, Luminex® human high-sensitivity cytokine immunoassay, Millipore human high-sensitivity cytokine panel

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About This Item

Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Livello qualitativo

Reattività contro le specie

human

Produttore/marchio commerciale

Milliplex®

assay range

accuracy: 98-107%
sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC)

standard curve range: 0.18-750 pg/mL
(IL-6)

standard curve range: 0.24-1,000 pg/mL
(IL-13)

standard curve range: 0.24-1,000 pg/mL
(IL-21)

standard curve range: 0.31-1,250 pg/mL
(IL-8)

standard curve range: 0.31-1,250 pg/mL
(MIP-1α)

standard curve range: 0.37-1,500 pg/mL
(IL-7)

standard curve range: 0.45-1,750 pg/mL
(TNFα)

standard curve range: 0.49-2,000 pg/mL
(IL-1β)

standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70))

standard curve range: 0.49-2,000 pg/mL
(IL-2)

standard curve range: 0.49-2,000 pg/mL
(IL-5)

standard curve range: 0.61-2,500 pg/mL
(IFNγ)

standard curve range: 0.61-2,500 pg/mL
(MIP-3α)

standard curve range: 0.73-3,000 pg/mL
(IL-17A)

standard curve range: 0.92-3,750 pg/mL
(MIP-1β)

standard curve range: 1.22-5,000 pg/mL
(GM-CSF)

standard curve range: 1.46-6,000 pg/mL
(IL-10)

standard curve range: 1.46-6,000 pg/mL
(ITAC)

standard curve range: 1.83-7,500 pg/mL
(IL-4)

standard curve range: 18.3-75,000 pg/mL
(Fractalkine)

standard curve range: 7.93-32,500 pg/mL
(IL-23)

inter-assay cv: <15%
intra-assay cv: <10%

tecniche

multiplexing: suitable

Metodo di rivelazione

fluorometric (Luminex xMAP)

Condizioni di spedizione

wet ice

Descrizione generale

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

The MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel is to be used for the simultaneous quantification of any or all of the following analytes in human plasma, serum, and cell/tissue culture supernatant samples: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, ITAC, MIP-1α, MIP-1β, MIP-3α
and TNFα.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificità

Cross Reactivty
There is no detectable cross-reactivity within the panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Applicazioni

  • Analytes: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, I-TAC, MIP-1α, MIP-1β, MIP-3α, TNF-α
  • Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay.
  • Recommended Sample type: Serum, plasma or tissue/cell lysate and culture supernatant
  • Assay Run Time: Overnight
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Immune Response, Metabolic Disorders, Chronic Inflammatory Disease, Cardiovascular Disease, Neuroscience

Caratteristiche e vantaggi

Design your multiplex kit by choosing available analytes within this panel.

Linkage

Replaces: HSTCMAG-28SK; HSCYTMAG-60SK

Stoccaggio e stabilità

Recommended storage for kit components is 2 - 8°C.

Altre note

Please contact Technical Service for linearity of dilution.
Sensitivity: Please see kit protocol for individual assay sensitivities.

Note legali

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Avvertenze

Danger

Classi di pericolo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Organi bersaglio

Respiratory Tract

Codice della classe di stoccaggio

10 - Combustible liquids


Certificati d'analisi (COA)

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Yu-Fei Gao et al.
International journal of ophthalmology, 12(11), 1708-1713 (2019-11-20)
To compare the anti-inflammatory effects of intense pulsed light (IPL) with tobramycin/dexamethasone plus warm compress through clinical signs and cytokines in tears. Eighty-two patients with dry eye disease (DED) associated meibomian gland dysfunction (MGD) were divided into two groups. Group
Bruna M Ascoli et al.
International journal of bipolar disorders, 7(1), 13-13 (2019-06-04)
Innate immune system dysfunction has been recognized as an important element in the pathophysiology of bipolar disorder (BD). We aimed to investigate whether there are differences in the response of macrophages derived from patients in the early stages and late
B Karshikoff et al.
Brain, behavior, and immunity, 57, 38-46 (2016-04-09)
Low-grade systemic inflammation has been implicated in chronic pain, as well as in comorbid diseases like depression and fatigue. We have previously shown that women's pain perception and regulation is more affected by systemic inflammation than that of men. Here
Joshua Y Lee et al.
Canadian journal of pain = Revue canadienne de la douleur, 5(1), 30-42 (2021-05-15)
Background: The prevalence of inadequate treatments for chronic pain has necessitated the search for biological factors that influence the transition to chronicity. Methods: Antecubital blood was drawn from those who experienced acute, noncatastrophic musculoskeletal trauma. Follow-up occurred at 1, 3
Amanda Priscila de Oliveira et al.
Cytokine, 91, 51-56 (2016-12-22)
The aim of this study was to investigate the plasma levels of the CCL3 and CCL4 chemokines in patients with the cardiac and digestive clinical forms of chronic Chagas disease and in cardiac patients with and without left ventricular systolic

Contenuto correlato

Immunology multiplex assays, like MILLIPLEX® assays, allow researchers to investigate more immune biomarkers simultaneously, saving time and resources. This is especially critical to immunology research, where numerous analytes play a role in different pathways.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.