P8651
Protein A–Peroxidase from Staphylococcus aureus/horseradish
lyophilized powder
Synonym(s):
Peroxidase–Protein A
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About This Item
Recommended Products
conjugate
peroxidase conjugate
Quality Level
100
200
form
lyophilized powder
technique(s)
direct ELISA: 1:80,000 using human IgG
storage temp.
−20°C
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General description
Protein A is a cell wall constituent of Staphylococcus aureus, with a molecular weight of 56kDa. It possesses five binding domains which interact with heavy chains of immunoglobulins at the Fc or Fab fragment. In response to protein A, the complement system, interferon production, hypersensitivity reactions and cell-mediated cytotoxicity get activated. Horseradish peroxidase is a heme-containing enzyme. In the presence of hydrogen peroxide, the enzyme catalyzes the oxidation of luminol.
Specificity
Binds IgG only from most mammals, except rat, goat, sheep.
Application
Protein A–Peroxidase from Staphylococcus aureus/horseradish has been used in enzyme-linked immunosorbent assay (ELISA).
Packaging
Package size based on protein content.
Physical form
Lyophilized powder containing sodium citrate buffer salt. Protein determined by A205.
Preparation Note
Protein A, extracellular, coupled to Peroxidase, Type VI, by a modification of the procedure of O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978), which favors low molecular weight conjugates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
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