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Test Author Name, MBBS, MD, Ph.D1,2, Test Author Name 2, Test Author Title 21, Test Author Name 3, Test Author Title 32

Westorn Bloting Princliple and Methods, Chapter 1

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Left Primary Content

  • Manoj Overridden Page
    Cellular assays, or cell-based assays, are essential tools for measuring cell health, viability, proliferation, migration, invasion, chemotaxis, apoptosis, angiogenesis, oxidative stress, cell signaling and live cell analysis.
  • Cell Counting & Health Analysis
    Techniques for counting cells and monitoring cell health and viability in culture, including calorimetric and fluorescent cell viability and proliferation assays. Methods for manual or automated cell counting include Coulter devices.
  • Imaging Analysis & Live Cell Imaging
    An overview of current tools and reagents for live cell imaging and analysis with applications in cell trafficking, cell health, gene expression analysis, cell migration, 3D cell culture, cytoskeletal dynamics and more.
  • Mammalian Cell Culture
    Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
  • Microbial Cell Culture
    An overview of microbiological cell culture and its applications in molecular biology, cloning, and recombinant protein expression as well as cultivation for the isolation, differentiation, and identification of disease-causing microbes in clinical researc
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Right Content Headline

  • Overridden Page Title
    Plant cell and tissue culture in commercial plant production, conservation, and plant research. Meristem culture, virus-free plant propagation, protoplast and haploid plant production.
  • Primary Cell Culture
    Primary cells isolated directly from human and animal sources maintain physiological relevance of the tissues from which they are derived, and are used for ADME/Tox screening. Find applications and methods for culturing mammalian primary cells in vitro.
  • Stem Cell Culture
    An overview of different stem cell types, stem cell culture basics and stem cell applications in basic research and clinical research applications, such as tissue regeneration, genetic diseases and cancer.
  • Cell Culture Media Preparation
    Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.
  • Cell Growth & Maintenance
    A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.
  • Transfection & Gene Editing
    Cell transfection principles, common transfection methods, and the role of transfection in gene editing and silencing for research, drug discovery, recombinant protein production.
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Reference Headline

1.
Given Family Name TGN. 2019. Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy. 3rd Edition. Bangalore: Elsevier.
2.
2019. Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy. 3rd Edition. NY: Elsevier.
3.
Given Family Name 2 GN2. 2019. Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy. 4th Edition. NJ: Elsevier.
4.
Given Family Name TGN. 2019. Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy. 4th Edition. Bangalore: Elsevier.
5.
Family Name 2 GN2. 2019. Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy. 5th Edition. NY: Elsevier.
6.
Family Name GN. 2020. Webpage Title. [Internet]. Bangalore: Bangalore Publication.[updated 28 Dec 2020; cited 28 Dec 2020]. Available from: https://www.google.com

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Slide 1 of 7
Overridden Page Title 1
Overridden Page Title 1
Atomic spectroscopy uses the energy absorbed or emitted by electrons to identify and quantify the elemental composition of a sample. It includes various analytical techniques, such as AAS, AES, FAA, GFAA, ICP-OES, ICP-MS and XRF.
Calibration, Qualification & Validation
Calibration, Qualification & Validation
Reference materials are critical to method validation, calibration, qualification, and measurement of uncertainty. The proper selection of the reference material best suited for the testing application is vital, as results are only as accurate as the reference.
Gas Chromatography (GC)
Gas Chromatography (GC)
Gas chromatography is a common analytic technique used to separate and analyze volatile compounds in the gas phase. GC is applied in many industries for quality control, and to identify and/or quantify compounds in a mixture.
Large Molecule HPLC
Large Molecule HPLC
High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.
Low Pressure Liquid Chromatography
Low Pressure Liquid Chromatography
Low pressure liquid chromatography (LPLC) is a chromatographic technique that operates at low pressures to drive the mobile phase onto the column containing a stationary phase by the action of a pump.
Small Molecule HPLC
Small Molecule HPLC
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
Related Content Grid Headline
Overridden Page Title
Cellular assays, or cell-based assays, are essential tools for measuring cell health, viability, proliferation, migration, invasion, chemotaxis, apoptosis, angiogenesis, oxidative stress, cell signaling and live cell analysis.
Cell Counting & Health Analysis
Techniques for counting cells and monitoring cell health and viability in culture, including calorimetric and fluorescent cell viability and proliferation assays. Methods for manual or automated cell counting include Coulter devices.
Imaging Analysis & Live Cell Imaging
An overview of current tools and reagents for live cell imaging and analysis with applications in cell trafficking, cell health, gene expression analysis, cell migration, 3D cell culture, cytoskeletal dynamics and more.
Mammalian Cell Culture
Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
Microbial Cell Culture
An overview of microbiological cell culture and its applications in molecular biology, cloning, and recombinant protein expression as well as cultivation for the isolation, differentiation, and identification of disease-causing microbes in clinical researc
Plant Tissue Culture
Plant cell and tissue culture in commercial plant production, conservation, and plant research. Meristem culture, virus-free plant propagation, protoplast and haploid plant production.
Primary Cell Culture
Primary cells isolated directly from human and animal sources maintain physiological relevance of the tissues from which they are derived, and are used for ADME/Tox screening. Find applications and methods for culturing mammalian primary cells in vitro.
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