HPLC Analysis of 2,3-Dihydroxy-3-Phenylpropionic Acid Enantiomers on CHIROBIOTIC R

column

CHIROBIOTIC R, 25 cm x 4.6 mm I.D., 5 μm particles (13024AST)

mobile phase

[A] 0.1% ammonia, pH 4.1 with formic acid; [B] methanol; (50:50, A:B)

flow rate

1 mL/min

column temp.

ambient

detector

UV, 258 nm

injection

10 μL

sample

2,3-dihydroxy-3-phenyl-propionic acid isomers:

(A) racemate A (4.85 and 6.95 min)

(B) racemate B (5.33 and 6.29 min)