Diacylglycerol kinase η1 is a high affinity isozyme for diacylglycerol.

Diacylglycerol kinase η1 is a high affinity isozyme for diacylglycerol.

FEBS letters (2015-04-12)

Suguru Komenoi, Fumika Takemura, Hiromichi Sakai, Fumio Sakane

ABSTRACT

Diacylglycerol kinase (DGK) η plays important roles in various patho-physiological events such as oncogenesis. In this study, we performed an enzymological characterization of DGKη splice variant 1 (DGKη1). The Km value for diacylglycerol was 0.14 mol%. Intriguingly, the Km value of DGKη1 for diacylglycerol was at least 9-fold lower than those of other DGK isozymes including DGKα, indicating that DGKη1 is a high affinity isozyme for diacylglycerol. Therefore, DGKη1 is a unique DGK isozyme, which may function at particular membrane sites where only low concentrations of diacylglycerol are supplied.

MATERIALS

Product Number

Brand

Product Description

M8266

Sigma-Aldrich

Magnesium chloride, anhydrous, ≥98%

M4880

Sigma-Aldrich

Magnesium chloride, BioReagent, suitable for insect cell culture, ≥97.0%

208337

Sigma-Aldrich

Magnesium chloride, powder, <200 μm

449164

Sigma-Aldrich

Magnesium chloride, AnhydroBeads^™, −10 mesh, 99.9% trace metals basis

449172

Sigma-Aldrich

Magnesium chloride, AnhydroBeads^™, −10 mesh, 99.99% trace metals basis

83264

Sigma-Aldrich

HEPES buffer solution, 1 M in H₂O

800819C

Avanti

18:0-22:6 DG, 1-stearoyl-2-docosahexaenoyl-sn-glycerol, chloroform

800742C

Avanti

N-20:4 L-Serine (ARA-S), N-arachidonoyl L-serine, chloroform

H4034

Sigma-Aldrich

HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

S5886

Sigma-Aldrich

Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

H6147

Sigma-Aldrich

HEPES, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

S8776

Sigma-Aldrich

Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture

H3375

Sigma-Aldrich

HEPES, ≥99.5% (titration)

S6150

Sigma-Aldrich

Sodium chloride, tablet

S3014

Sigma-Aldrich

Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

M8787

Sigma-Aldrich

Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂solution for PCR

M1028

Sigma-Aldrich

Magnesium chloride solution, for molecular biology, 1.00 M±0.01 M

S6546

Sigma-Aldrich

Sodium chloride solution, 5 M

S5150

Sigma-Aldrich

Sodium chloride solution, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture

S1679

Sigma-Aldrich

Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

S7653

Sigma-Aldrich

Sodium chloride, BioXtra, ≥99.5% (AT)

H7523

Sigma-Aldrich

HEPES, BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)

450006

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

54457

Sigma-Aldrich

HEPES, BioUltra, for molecular biology, ≥99.5% (T)

68475

Sigma-Aldrich

Magnesium chloride solution, BioUltra, for molecular biology, 2 M in H₂O

D0138

Sigma-Aldrich

1,2-Dioleoyl-sn-glycerol, ≥97%

71386

Sigma-Aldrich

Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H₂O

63036

Sigma-Aldrich

Magnesium chloride solution, BioUltra, for molecular biology, ~0.025 M in H₂O

71376

Sigma-Aldrich

Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)

43816

Sigma-Aldrich

DL-Dithiothreitol solution, BioUltra, for molecular biology, ~1 M in H₂O