- Mycobacterium tuberculosis nitrogen assimilation and host colonization require aspartate.
Mycobacterium tuberculosis nitrogen assimilation and host colonization require aspartate.
Nature chemical biology (2013-10-01)
Alexandre Gouzy, Gérald Larrouy-Maumus, Ting-Di Wu, Antonio Peixoto, Florence Levillain, Geanncarlo Lugo-Villarino, Jean-Luc Guerquin-Kern, Jean-Luc Gerquin-Kern, Luiz Pedro Sório de Carvalho, Yannick Poquet, Olivier Neyrolles
PMID24077180
ABSTRACT
Here we identify the amino acid transporter AnsP1 as the unique aspartate importer in the human pathogen Mycobacterium tuberculosis. Metabolomic analysis of a mutant with an inactive AnsP1 revealed that the transporter is essential for M. tuberculosis to assimilate nitrogen from aspartate. Virulence of the AnsP1 mutant is impaired in vivo, revealing that aspartate is a primary nitrogen source required for host colonization by the tuberculosis bacillus.
MATERIALS
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Product Description
Sigma-Aldrich
L-Aspartic acid, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Supelco
L-Aspartic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland