Biosynthesis of silver nanoparticles from Trichoderma species.

Biosynthesis of silver nanoparticles from Trichoderma species.

Indian journal of experimental biology (2013-08-01)

T Prameela Devi, S Kulanthaivel, Deeba Kamil, Jyoti Lekha Borah, N Prabhakaran, N Srinivasa

ABSTRACT

A total of 75 isolates belonging to five different species of Trichoderma viz., T. asperellum, T. harzianum, T. longibrachiatum, T. pseudokoningii and T. virens were screened for the production of silver nanoparticles. Although all the isolates produced nanoparticles, T. virens VN-11 could produce maximum nanoparticles as evident from the UV-Vis study. The highest Plasmon band was observed at 420 nm at every 24 h that attained maximum intensity at 120 h (0.543). The high resolution transmission electron microscopy (HRTEM) further provided the morphology of the nanoparticles. These nanoparticles were found single or aggregated with round and uniform in shape and 8-60 nm in size. The nitrate reductase activity of VN-11 was found to be 150 nmol/h/mL which confirmed the production of silver nanoparticles through reduction of Ag+ to Ag0.

MATERIALS

Product Number

Brand

Product Description

85131

Sigma-Aldrich

Silver, colloidal, 65-75% Ag basis

265500

Sigma-Aldrich

Silver, powder, <250 μm, 99.99% trace metals basis

265551

Sigma-Aldrich

Silver, wire, diam. 0.127 mm, 99.99% trace metals basis

265527

Sigma-Aldrich

Silver, foil, thickness 0.1 mm, 99.9% trace metals basis

265608

Sigma-Aldrich

Silver, wire, diam. 1.0 mm, 99.9% trace metals basis

265586

Sigma-Aldrich

Silver, wire, diam. 0.5 mm, ≥99.99% trace metals basis

295744

Sigma-Aldrich

Silver, wool, diam. 0.05 mm, ≥99.9% trace metals basis

265594

Sigma-Aldrich

Silver, wire, diam. 1.0 mm, ≥99.99% trace metals basis

327050

Sigma-Aldrich

Silver, shot, 1-3 mm, ≥99.99% trace metals basis

484059

Sigma-Aldrich

Silver, nanopowder, <150 nm particle size, 99% trace metals basis

369438

Sigma-Aldrich

Silver, foil, thickness 1.0 mm, 99.99% trace metals basis

348783

Sigma-Aldrich

Silver, wire, diam. 0.1 mm, 99.9% trace metals basis

326976

Sigma-Aldrich

Silver, foil, thickness 2.0 mm, 99.9% trace metals basis

739448

Sigma-Aldrich

Silver nanowires, diam. × L 100 nm × 6 μm, 0.5% (isopropyl alcohol suspension)

739421

Sigma-Aldrich

Silver nanowires, diam. × L 50 nm × 6 μm, 0.5% (isopropyl alcohol suspension)

735825

Sigma-Aldrich

Silver, conductive paste

265578

Sigma-Aldrich

Silver, wire, diam. 0.25 mm, 99.9% trace metals basis

265519

Sigma-Aldrich

Silver, foil, thickness 0.025 mm, 99.9% trace metals basis

267430

Sigma-Aldrich

Silver, wire, diam. 2.0 mm, ≥99.99% trace metals basis

373249

Sigma-Aldrich

Silver, evaporation slug, diam. × L 0.6 cm × 1.2 cm, 99.99% trace metals basis

303372

Sigma-Aldrich

Silver, granular, >250 μm, 99.99% trace metals basis

348759

Sigma-Aldrich

Silver, wire, diam. 1.5 mm, ≥99.99% trace metals basis

327034

Sigma-Aldrich

Silver, wire, diam. 0.25 mm, ≥99.99% trace metals basis

326984

Sigma-Aldrich

Silver, foil, thickness 0.25 mm, 99.9% trace metals basis

327018

Sigma-Aldrich

Silver, wire, diam. 2.0 mm, 99.9% trace metals basis

327026

Sigma-Aldrich

Silver, wire, diam. 0.5 mm, 99.9% trace metals basis

327085

Sigma-Aldrich

Silver, powder, 2-3.5 μm, ≥99.9% trace metals basis

327107

Sigma-Aldrich

Silver, powder, <45 μm, ≥99.99% trace metals basis

327093

Sigma-Aldrich

Silver, powder, 5-8 μm, ≥99.9% trace metals basis

327077

Sigma-Aldrich

Silver, flakes, 10 μm, ≥99.9% trace metals basis