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Merck
  • Deuterated matrix-assisted laser desorption ionization matrix uncovers masked mass spectrometry imaging signals of small molecules.

Deuterated matrix-assisted laser desorption ionization matrix uncovers masked mass spectrometry imaging signals of small molecules.

Analytical chemistry (2012-08-07)
Mohammadreza Shariatgorji, Anna Nilsson, Richard J A Goodwin, Per Svenningsson, Nicoletta Schintu, Zoltan Banka, Laszlo Kladni, Tibor Hasko, Andras Szabo, Per E Andren
ABSTRACT

D(4)-α-Cyano-4-hydroxycinnamic acid (D(4)-CHCA) has been synthesized for use as a matrix for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) and MALDI-MS imaging (MSI) of small molecule drugs and endogenous compounds. MALDI-MS analysis of small molecules has historically been hindered by interference from matrix ion clusters and fragment peaks that mask signals of low molecular weight compounds of interest. By using D(4)-CHCA, the cluster and fragment peaks of CHCA, the most common matrix for analysis of small molecules, are shifted by + 4, + 8 and + 12 Da, which expose signals across areas of the previously concealed low mass range. Here, obscured MALDI-MS signals of a synthetic small molecule pharmaceutical, a naturally occurring isoquinoline alkaloid, and endogenous compounds including the neurotransmitter acetylcholine have been unmasked and imaged directly from biological tissue sections.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
α-Cyano-4-hydroxycinnamic acid, suitable for MALDI-TOF MS
Sigma-Aldrich
α-Cyano-4-hydroxycinnamic acid, ≥98% (TLC), powder
Sigma-Aldrich
α-Cyano-4-hydroxycinnamic acid, matrix substance for MALDI-MS, Ultra pure
Supelco
α-Cyano-4-hydroxycinnamic acid, matrix substance for MALDI-MS, ≥99.0% (HPLC)
Sigma-Aldrich
α-Cyano-4-hydroxycinnamic acid, 97%
Sigma-Aldrich
α-Cyano-4-hydroxycinnamic acid, 99%