- Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.
Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.
Applied and environmental microbiology (2010-04-20)
Akos T Kovács, Mariska van Hartskamp, Oscar P Kuipers, Richard van Kranenburg
PMID20400555
ABSTRACT
Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
D-Lactic Dehydrogenase from Lactobacillus leichmannii, ammonium sulfate suspension, ≥250 units/mg protein (biuret)
Sigma-Aldrich
D-Lactic Dehydrogenase from Lactobacillus leichmannii, lyophilized powder, 150-500 units/mg protein
Sigma-Aldrich
D-Lactic Dehydrogenase from Staphylococcus epidermidis, lyophilized powder, ≥80 units/mg solid