- Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.
Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.
Science (New York, N.Y.) (1985-05-17)
G G Wong, J S Witek, P A Temple, K M Wilkens, A C Leary, D P Luxenberg, S S Jones, E L Brown, R M Kay, E C Orr
PMID3923623
ABSTRACT
Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
GM-CSF from mouse, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sigma-Aldrich
Granulocyte-Macrophage Colony-Stimulating Factor human, GM-CSF, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture