N3129

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

• Synonym(s): p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
• Linear Formula: O₂NC₆H₄OP(O)(O^-)₂ · 2C₆H₁₁NH₃⁺
• CAS Number: 52483-84-8
• Molecular Weight: 417.44
• Beilstein: 6553276
• EC Number: 257-949-0
• MDL number: MFCD00013173
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897623
• NACRES: NA.83

Properties

• product name: 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate
• Quality Level: 300
• Assay: ≥97% anhydrous basis
• form: powder
• solubility: water: 10 mg/mL, clear, colorless to faintly yellow
• storage temp.: −20°C
• SMILES string: NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
• InChI key: KXNNUYCXODNFBD-UHFFFAOYSA-N

Description

Application

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves