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Key Documents

GF136

Sigma-Aldrich

BAFF Protein, Recombinant human

The Human BAFF protein (B-cell activating factor belonging to the TNF family) is a novel ligand in the TNF family.

Synonym(s):

B-cell Activation Factor, TALL-1, THANK, BLyS

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77

biological source

human

Quality Level

Assay

>98% (SDS-PAGE)

mol wt

Mw 17.0 kDa

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

impurities

<0.1 ng/μg Endotoxin (of BAFF; 1 EU/ug)

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

Human BAFF (B-cell activating factor belonging to the TNF family) is a novel ligand in the TNF family. BAFF plays an important role as co-stimulator of B-cell proliferation and function. Recombinant human BAFF is a soluble 17.0 kDa protein containing 153 amino acid residues, comprising the TNF-like extracellular domain of BAFF.
Product Source: E.coli

Physical form

Lyophilized from sterile filtered 0.5X PBS, pH 7.5, and 0.5 mM DTT, containing no additives.

Storage and Stability

The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C for up to one year from date of receipt. Reconstituted material can be stored at 2°-8°C for one week, but undiluted aliquots at -20°C are recommended. Avoid repeated freeze/thaw cycles.

Analysis Note

Specific Activity: Human BAFF is biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on human RPMI 8226 cells is 5-10 ng/mL.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

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