HPLC Analysis of Warfarin Enantiomers on Astec^® CHIROBIOTIC^® V2

column

CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)

mobile phase

[A] 10 mM ammonium acetate, pH 4.1; [B] acetonitrile; (70:30, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

10 μL

sample

1 mg/mL in methanol