HPLC Analysis of Cosmetic Preservatives on Ascentis® Express RP-Amide
Materials
analytical column
Product No.
Description
Pricing
standard
CONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 3 mm I.D., 2.7 μm particles (53918-U)
mobile phase
[A] water; [B] methanol; (60:40, A:B)
flow rate
0.6 mL/min
pressure
2900 psi (200 bar)
column temp.
50 °C
detector
UV, 250 nm
injection
1 μL
sample
methyl-, benzylparaben (50 mg/L), ethylparaben (30 mg/L), propyl-, butylparaben (100 mg/L), 2-phenoxyethanol (1.5 g/L),1-phenoxy-2-propanol (3 g/L), all in 40% methanol
Description
Analysis Note
With the publication Borremans, M. et.al. 2004, Chromatographia 59: 47-53 as a reference, this method is 10% faster, but with superior resolution of critical pairs; in this case, the minimum USP resolution is 2.4.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany