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HPLC Analysis of Ascorbic Acid on Ascentis® RP-Amide

HPLC Analysis of Ascorbic Acid on Ascentis® RP-Amide application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

25 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 230 nm

injection

10 μL

sample

as indicated each in mobile phase

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany