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  • The utilization of 3-mercapto-2-methylpropionate as sulphur source by a phototrophic bacterium.

The utilization of 3-mercapto-2-methylpropionate as sulphur source by a phototrophic bacterium.

Bioorganic & medicinal chemistry (1994-07-01)
M R Smith, W J van den Tweel, J A de Bont
ABSTRACT

A bacterium (strain photoB) photoassimilated 3-mercapto-2-methylpropionate as sole source of sulphur with methacrylate accumulating in the medium. This was thought to be the product of a sulphur-lyase type enzyme attacking the 3-mercapto-2-methylpropionate. Detailed examination of the biochemistry of the utilization of 3-mercapto-2-methylpropionate showed however that the thiol was first activated to 3-mercapto-2-methylpropionyl CoA. It appeared that this CoA-derivative then served as the substrate for a sulphur-lyase type enzyme, with methacrylyl-CoA as product. Further metabolism was via beta-hydroxyisobutyryl-CoA and beta-hydroxyisobutyrate.

MATERIALS
Product Number
Brand
Product Description

Captopril impurity C, European Pharmacopoeia (EP) Reference Standard