Isotope-based analysis of modified tRNA nucleosides correlates modification density with translational efficiency.

Isotope-based analysis of modified tRNA nucleosides correlates modification density with translational efficiency.

Angewandte Chemie (International ed. in English) (2012-10-06)

Caterina Brandmayr, Mirko Wagner, Tobias Brückl, Daniel Globisch, David Pearson, Andrea Christa Kneuttinger, Veronika Reiter, Antje Hienzsch, Susanne Koch, Ines Thoma, Peter Thumbs, Stylianos Michalakis, Markus Müller, Martin Biel, Thomas Carell

PMID23037940

ABSTRACT

Useful diversity: Quantification of modified tRNA nucleobases in different murine and porcine tissues reveals a tissue-specific overall modification content. The modification content correlates with rates of protein synthesis in vitro, suggesting a direct link between tRNA modification levels and tissue-specific translational efficiency.

MATERIALS

Product Number

Brand

Product Description

R5125

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

R4875

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

R6513

Sigma-Aldrich

Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

R5250

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

R5000

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein

R7884

Sigma-Aldrich

Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)

R5503

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein

R4642

Sigma-Aldrich

Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

R5500

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein