I8782
Interferon αA from mouse
≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture
Synonym(s):
IFN-αA
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About This Item
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biological source
mouse
Quality Level
recombinant
expressed in E. coli
Assay
≥95%
form
buffered aqueous solution
mol wt
20 kDa
technique(s)
cell culture | mammalian: suitable
color
colorless
shipped in
dry ice
storage temp.
−70°C
Gene Information
mouse ... Ifna3(110218)
Biochem/physiol Actions
Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities. Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes) and ruminant embryos (τ subtypes). Interferon induces a variety of effects on target cells including antiviral, antiproliferative and immunomodulatory activities.
Packaging
Minimum 1x105 units/vial.
Physical form
Solution in phosphate buffered saline containing 0.1% bovine serum albumin.
Analysis Note
The biological activity is determined in the cytopathic effect inhibition assay using mouse L929 cells with encephalomyocarditis virus. The cytopathic effect of 50% is produced with approximately 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for mouse interferon α.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Interferon standards and general abbreviations.
S Pestka
Methods in enzymology, 119, 14-23 (1986-01-01)
A convenient and rapid cytopathic effect inhibition assay for interferon.
P C Familletti et al.
Methods in enzymology, 78(Pt A), 387-394 (1981-01-01)
S Rubinstein et al.
Journal of virology, 37(2), 755-758 (1981-02-01)
A convenient assay for interferons based on reduction of cytopathic effect was developed. The number of manipulations and the lengths of the various incubation steps were reduced to a minimum. The assay is simple to perform and can be completed
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