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product name
DK, 93120836
biological source
dog kidney
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Canine kidney
Cell Line Description
Derived from the kidney cortex of a 6 week old Beagle of unknown sex. The cells support the growth of many viruses including influenza, parainfluenza-2, infectious bovine rhinotracheitis, canine hepatitis, adenovirus, vaccinia and VSV (Indiana strain).
Application
DK cell line has been used to study the functional organization of the major late transcriptional unit of canine adenovirus type 2 (CAV-2).
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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