MABT1498

Anti-ZDHHC13 Antibody, clone 26D1

clone 26D1, from mouse

• Synonym(s): Palmitoyltransferase ZDHHC13, EC: 2.3.1.22, Huntingtin-interacting protein 14-related protein, HIP14-related protein, Huntingtin-interacting protein HIP3RP, Putative MAPK-activating protein PM03, Putative NF-kappa-B-activating protein 209, Zinc finger DH
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 26D1, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μL
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001001483
• UniProt accession no.: Q8IUH4-2
• target post-translational modification: unmodified
• Gene Information: human ... ZDHHC13(54503)

Description

General description

Palmitoyltransferase ZDHHC13 (UniProt: Q8IUH4; also known as EC: 2.3.1.22, Huntingtin-interacting protein 14-related protein, HIP14-related protein, Huntingtin-interacting protein HIP3RP, Putative MAPK-activating protein PM03, Putative NF-kappa-B-activating protein 209, Zinc finger DHHC domain-containing protein 13, DHHC-13) is encoded by the ZDHHC13 (also known as HIP14L, HIP3RP) gene (Gene ID: 54503) in human. DHHC-13 is a multi-pass membrane protein that serves as a palmitoyl acyltransferase (PAT) specific for a subset of neuronal proteins, including HD and GAD2 and may play a role in the pathogenesis of Huntington disease (HD). It is reported to be dysfunctional in the presence of the HD mutation and HIP-14 deficient mice are shown to develop features of HD. DHHC-13 also plays a role in cellular magnesium homeostasis. It contains a DHHC domain (aa 426-476 in isoform 1 and 250-300 in isoform 2), which is required for its palmitoyltransferase activity. (Ref.: Young, FB et al. (2011). Prog. Neurobiol. 97(2); 220-238).

Specificity

Clone 26D1 detects human Palmitoyltransferase ZDHHC13. It targets an epitope within 111 amino acids from the N-terminal region of isoforms 1 and 2. This sequence is conserved in both isoforms.

Immunogen

MBP-tagged recombinant fragment corresponding to 111 amino acids from the N-terminal region of human Palmitoyltransferase ZDHHC13, isoform 2.

Application

Anti-ZDHHC13, clone 26D1, Cat. No. MABT1498, is a mouse monoclonal antibody that detects Palmitoyltransferase ZDHHC13 and has been tested for use in Western Blotting.

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ZDHHC13 in A431 cell lysate.

Target description

~55 kDa observed; 56.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable