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71546

Sigma-Aldrich

BacMagic Transfection Kit - Novagen

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.54

manufacturer/tradename

Novagen®

Quality Level

100

storage condition

OK to freeze

General description

The BacMagic-1 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac, pBAC, or pTriEx vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic-1 Transfection Kit contains the BacMagic-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector Medium, for optimal transfection performance.

Components

  • 0.5 µg : BacMagic DNA
  • 50 µl : Insect GeneJuice® Transfection Reagent
  • 2 x 1 ml : Sf9 Cells
  • 1 L : BacVector Insect Cell Medium
  • 2 µg L : Transfection Control Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS

Storage and Stability

Ship Code: Multiple Storage ConditionsStorage: Multiple storage requirements

Legal Information

GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

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