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382158

Sigma-Aldrich

Anti-Histone H3, Acetylated (1-20) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

Tetrahymena sp., human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

General description

Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation.
Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein.
Recognizes the ~17 kDa acetylated histone H3 protein.

Immunogen

Human
a diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH

Application

Immunoblotting (10-500 ng/ml, chemiluminescence)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HeLa cells

Other Notes

Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349.
Braunstein, M., et al. 1993. Genes Devel.7, 592.
Perry, C.A., et al. 1993. Biochemistry32, 13605.
Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
For immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

    • Certificates of Analysis (COA)

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    Xingang Yuan et al.
    Molecular medicine reports, 14(2), 1139-1145 (2016-06-10)
    The aim of the present was to evaluate the effects of DNA methylation and histone acetylation on 2,3,7,8‑tetrachlorodibenzo‑p‑dioxin (TCDD)‑induced cleft palate in fetal mice. Pregnant mice (n=10) were randomly divided into two groups: i) TCDD group, mice were treated with 28 µg/kg
    Atsushi Kuno et al.
    Scientific reports, 8(1), 15555-15555 (2018-10-24)
    Autophagy activation improves the phenotype in mdx mice, a Duchenne muscular dystrophy (DMD) model, although the underlying mechanisms are obscure. We previously found that resveratrol, a strong inducer of autophagy, ameliorates the cardiac pathology of mdx mice. Autophagy could eliminate
    Matías Alvarez-Saavedra et al.
    Nature communications, 5, 4181-4181 (2014-06-21)
    Chromatin compaction mediates progenitor to post-mitotic cell transitions and modulates gene expression programs, yet the mechanisms are poorly defined. Snf2h and Snf2l are ATP-dependent chromatin remodelling proteins that assemble, reposition and space nucleosomes, and are robustly expressed in the brain.

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