05-716-I
Anti-Myosin Heavy Chain Antibody, clone A4.1025
clone A4.1025
Synonym(s):
MyHC-2x, MyHC-IIx/d, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d, Myosin heavy chain skeletal muscle adult 1, Myosin-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
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General description
Myosin-1 (UniProt: P12882; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain IIx/d, MyHC-IIx/d, Myosin heavy chain skeletal muscle adult 1) is encoded by the MYH1 gene (Gene ID: 4619) in human. Myosins are highly conserved, ubiquitously expressed proteins that form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Along with actin they serve as motor molecules with ATPase activity that is essential for muscle contraction. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). The globular head of myosin heavy chain contains the actin-activated ATPase. It has been reported that during rest, the myosin head does interact with the actin filament due to the presence of tropomyosin. However, when muscle contracts, tropomyosin shifts out of the way to allow the globular head to engage the actin filaments. Myosin heavy chain is a conventional myosin with a N-terminal SH3-like domain (aa 37-82), a myosin motor domain (aa 86-782), and an actin-binding domain (aa 761-775). It has a rod like tail sequence that is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils
Specificity
Clone A4.1025 is a mouse monoclonal antibody that detects Myosin Heavy Chain.
Immunogen
Partially purified myosin from adult human skeletal muscle.
Application
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Myosin Heavy Chain in lysates from L6 cells and human skeletal muscle.
mmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Myosin Heavy Chain in human skeletal muscle tissue sections.
Immunohistochemistry Applications: A representative lot detected Myosin Heavy Chain in Immunohistochemistry applications
Western Blotting Analysis: A representative lot detected Myosin Heavy Chain in Western Blotting applications
Immunofluorescence Analysis: A representative lot detected Myosin Heavy Chain in Immunofluorescence applications
Immunocytochemistry Analysis: A representative lot detected Myosin Heavy Chain in Immunocytometry applications
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
mmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Myosin Heavy Chain in human skeletal muscle tissue sections.
Immunohistochemistry Applications: A representative lot detected Myosin Heavy Chain in Immunohistochemistry applications
Western Blotting Analysis: A representative lot detected Myosin Heavy Chain in Western Blotting applications
Immunofluorescence Analysis: A representative lot detected Myosin Heavy Chain in Immunofluorescence applications
Immunocytochemistry Analysis: A representative lot detected Myosin Heavy Chain in Immunocytometry applications
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Quality
Evaluated by Western Blotting in C2C12 cell lysate.
Reconstitution
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Stable for 1 year at +2°C to +8°C from date of receipt.
Other Notes
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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