807745

APN-COCI

• Synonym(s): 4-(cyanoethynyl)benzoyl chloride
• Empirical Formula (Hill Notation): C₁₀H₄ClNO
• Molecular Weight: 189.60
• UNSPSC Code: 12352101
• PubChem Substance ID: 329768747
• NACRES: NA.22

Properties

• form: powder
• Quality Level: 100
• reaction suitability: reagent type: cross-linking reagent
• storage temp.: 2-8°C
• SMILES string: N#CC#CC1=CC=C(C(Cl)=O)C=C1
• InChI: 1S/C10H4ClNO/c11-10(13)9-5-3-8(4-6-9)2-1-7-12/h3-6H
• InChI key: OWNJCJKKKZXTIX-UHFFFAOYSA-N

Description

Application

APN-COCl is a bifunctional reagent appropriate for the introduction of APN function.

Other Notes

The coupling between an amine and APN-COCl should be performed in dry organic solvents (e.g. THF) in the presence of a base (e.g. TEA). The resulting conjugate can be readily coupled with thiol-containing biomolecules using the following protocol:

• Dissolve the protein in the appropriate buffer with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the APN-labeled molecule (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.

If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302,H314
• Precautionary Statements: P260 - P270 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Corr. 1B
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable