Protein Purification

Numerous protein purification methods are widely used in both biological and biomedical research. Recombinant protein expression and purification workflows depend on many variables. These variables include but are not limited to the physical properties and biological function of the protein, and whether a bacterial or eukaryotic cell line should be used to express the protein of interest. Significant advancements have been made in the area of recombinant protein expression  and purification methodology along with a plethora of commercially available systems and kits. However, proteins are complex macromolecules, and optimal protein expression and purification strategies must be determined empirically.