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RAB0224

Sigma-Aldrich

Mouse IFN-γ ELISA Kit

for serum, plasma, cell culture supernatant

Sinónimos:

IFN-gamma, Interferon gamma

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Ifng(15978)

General description

The Mouse IFN-γ ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IFN-γ in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Mouse IFN-γ

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse IFN-γ ELISA Kit has been used to measure serum proinflammatory cytokine levels in a renal ischemia/reperfusion injury model.

Biochem/physiol Actions

Interferon γ (IFN-γ) regulates cell differentiation and inflammatory responses. It stimulates the expression of apoptotic mediators. IFN-γ is associated with rheumatoid arthritis (RA). It reduces the differentiation of T helper (Th) 17 cells and osteoclasts. IFN-γ has immunomodulatory and antiviral activity. It is associated with autoimmune pathology. IFN-γ provides protection against tumor development.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis
Lee SH, et al.
Scientific Reports, 7(1), 10133-10133 (2017)
Interferon-gamma: an overview of signals, mechanisms and functions
Schroder K, et al.
Journal of Leukocyte Biology, 75(2), 163-189 (2004)
Qianqian Chen et al.
Journal of photochemistry and photobiology. B, Biology, 216, 112152-112152 (2021-02-21)
Emerging evidence suggests that the gut microbiota plays an important role in the pathological progression of Alzheimer's disease (AD). Photobiomodulation (PBM) therapy is believed to have a positive regulatory effect on the imbalance of certain body functions, including inflammation, immunity
Gypenoside attenuates renal ischemia/reperfusion injury in mice by inhibition of ERK signaling.
Ye Q, et. al.
Experimental and Therapeutic Medicine, 11(4), 1499-1505 (2016)
Kaveh Abdi et al.
Journal of immunology (Baltimore, Md. : 1950), 192(12), 6028-6036 (2014-05-14)
IL-12p40 partners with the p35 and p19 polypeptides to generate the heterodimeric cytokines IL-12 and IL-23, respectively. These cytokines play critical and distinct roles in host defense. The assembly of these heterodimers is thought to take place within the cell
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