CHO/dhFr-AC-free cell line
5011002, hamster ovary, Suspension
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About This Item
UNSPSC Code:
41106514
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product name
CHO/dhFr-AC-free cell line, 05011002
biological source
hamster ovary
growth mode
Aggregates in suspension
karyotype
Not specified
morphology
Suspension
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Hamster Chinese Ovary, serum-free
Cell Line Description
This is a derviative of the CHO/dhFr- cell line, Sigma Catalogue number. 94060607 which has been adapted to grow in the animal component free medium CHO DHFR (Sigma C8862). The CHO/dhFr- cell line lacks the enzyme dihydrofolate reductase (DHFR) which is necessary for purine synthesis. In the absence of exogenous purines, this enzyme is required for growth. Hence the CHO/dhFr- cell line has growth requirements for hypoxanthine (or adenine), glycine and thymidine. However, the cells will continue to grow without the addition of hypoxanthine and thymidine because the cells can obtain sufficient from the medium. α MEM minus does not contain the ribonucleosides or the deoxyribonucleosides, and will not support the growth of CHO/dhFr- or its serum free adaptation as neither contains the gene to produce DHFR.
Culture Medium
CHO DHR medium (Sigma C8862) + 2mM Glutamine + 100uM Hypoxanthine + 16uM Thymidine (e.g. use HT supplement Sigma H0137). Addition of Aminopterin (Sigma A5159) 0.4uM is also advised. When freezing cells use freeze medium composed of 50:50 fresh:conditioned medium + 10% DMSO.
Subculture Routine
Maintain cultures between approximately 3-9x105 cells/ml; 5% CO2; 37°C. Cells grow as aggregates in suspension. Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks.
Other Notes
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