525295

PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20)

liquid, clone PY20, Calbiochem^®

• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: PY20, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity (predicted by homology): all
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG2b
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pTyr)
• Gene Information: human ... PID1(55022)

Descripción

General description

Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.

Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.

This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.

Immunogen

KLH conjugated to phosphotyrosine, glycine, and alanine

Application

ELISA (see comments)

Immunoblotting (1:500-1:1000, see application references)

Frozen Sections (see application references)

Immunocytochemistry (see application references)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM sodium phosphate, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Any cell lysate or tissue extract

Other Notes

Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable