521225

PDGF, BB Homodimer, Human, Recombinant, E. coli

• Sinónimos: rhPDGF-BB
• UNSPSC Code: 12352202

Propiedades

• assay: ≥95% (SDS-PAGE)
• Quality Level: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤1 ng/μg Endotoxin (ng/μg PDGF-BB)
• shipped in: dry ice
• storage temp.: −70°C

Descripción

General description

Recombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal and mononuclear cells. Has the same amino acid sequence as mature human PDGF-B. Rapidly activates protein kinase D in vascular smooth muscle cells.

Recombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal, and mononuclear cells. PDGF represents one of the major mitogens present in platelets. It exerts its effects by binding to specific high-affinity dimeric receptor tyrosine kinases. Material has the same amino acid sequence as mature human PDGF-B. Two distinct PDGF receptors have been identified: the PDGFα receptor, which binds to all three forms of PDGF, and the PDGFβ receptor, which can bind only PDGF-BB and PDGF-AB.

Biochem/physiol Actions

ED₅₀ = 1-5 ng/ml as determined by the dose dependent proliferation of BALB/c 3T3 cells

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from a sterile-filtered solution, carrier free.

Reconstitution

Reconstitute to a concentration of ≥100 µg/ml 100 mM acetic acid, 0.1% BSA. Further dilute with low endotoxin medium or buffered solution containing FBS or tissue culture grade BSA just prior to use.

Other Notes

Abedi, H., et al. 1998. FEBS Lett. 427, 209.
Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
Westermark, B., and Heldin, C.-H. 1991. Cancer Res.51, 5087.
Raines, E.W., et al. 1985. Methods Enzymol.109, 749.
Johnson, A., et al. 1984. EMBO J.3, 921.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable