16-193
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate
clone JBW301, Upstate®, from mouse
Sinónimos:
H2AXS139P, Histone H2A.X (phospho S139), H2A histone family, member X, H2AX histone
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
JBW301, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer139)
Gene Information
human ... H2AX(3014)
General description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Specificity
A broad species cross-reactivity is expected based on conservation of sequence homology.
Recognizes Histone H2A.X phosphorylated at Ser139, Mr 15kDa.
Immunogen
Epitope: Ser139
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
Application
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate is a Mouse Monoclonal Antibody for detection of phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone & has been validated in ICC & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Quality
Routinely evaluated in acid extracted histone from Jurkat cell lysates treated with 0.5mM staurosporine (19-123).
Western Blot Analysis:
0.5-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5μM staurosporine (Catalog # 19-123).
Western Blot Analysis:
0.5-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5μM staurosporine (Catalog # 19-123).
Target description
15 kDa
Physical form
Protein G Purified
Purified biotin conjugated mouse monoclonal IgG1 in buffer containing 70% storage buffer (0.02 M Phosphate buffer, pH7.6, 0.25 M NaCl, 0.1% sodium azide) and 30% glycerol
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Analysis Note
Control
Staurosporine-treated Jurkat cell lysates.
Staurosporine-treated Jurkat cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Sascha Schäuble et al.
PloS one, 7(8), e42150-e42150 (2012-08-11)
Primary human fibroblasts in tissue culture undergo a limited number of cell divisions before entering a non-replicative "senescent" state. At early population doublings (PD), fibroblasts are proliferation-competent displaying exponential growth. During further cell passaging, an increasing number of cells become
Christophe E Redon et al.
Methods in molecular biology (Clifton, N.J.), 682, 249-270 (2010-11-09)
Measurement of DNA double-strand break (DSB) levels in cells is useful in many research areas, including those related to DNA damage and repair, tumorigenesis, anti-cancer drug development, apoptosis, radiobiology, environmental effects, and aging, as well as in the clinic. DSBs
Lars H Jensen et al.
BMC pharmacology, 4, 31-31 (2004-12-04)
Bisdioxopiperazine anti-cancer agents are inhibitors of eukaryotic DNA topoisomerase II, sequestering this protein as a non-covalent protein clamp on DNA. It has been suggested that such complexes on DNA represents a novel form of DNA damage to cells. In this
Hélène Royo et al.
PLoS genetics, 11(10), e1005461-e1005461 (2015-10-29)
During the pachytene stage of meiosis in male mammals, the X and Y chromosomes are transcriptionally silenced by Meiotic Sex Chromosome Inactivation (MSCI). MSCI is conserved in therian mammals and is essential for normal male fertility. Transcriptomics approaches have demonstrated
J M Cloutier et al.
Chromosoma, 125(2), 215-226 (2015-12-30)
During mammalian meiotic prophase I, surveillance mechanisms exist to ensure that germ cells with defective synapsis or recombination are eliminated, thereby preventing the generation of aneuploid gametes and embryos. Meiosis in females is more error-prone than in males, and this
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico