PEPscreen Dissolution, Storage and Handling Guidelines
Complete solubilization of peptides is important for successful screening of peptide activity. Peptides can be fully active only if they are completely solubilized and are able to assume the correct conformation for binding to their receptors. As the number of peptides in a set increase, so does the potential solubility variation of the peptides within the set. Therefore, in order to obtain accurate and reliable peptide activity data, careful attention should be devoted to the process of dissolving peptide library sets. The strategy for dissolving the peptide set is different from dissolving individual peptides. For individual peptides, conditions are chosen for optimum solubility based on the given peptide sequence. However, for peptide sets (or peptide libraries) conditions are chosen to dissolve as many of the peptides in the set as possible in the first solubilization attempt.
Below is a quick reference for peptide dissolution, storage and handling of peptide library sets.
Peptide Library Dissolution
Figure 1.A streamlined approach for dissolving a large number of peptides
PEPTIDE LIBRARY STORAGE GUIDELINES: |
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Peptide Library Handling Guidelines
- Keep the peptide-containing vial sealed as much as possible
- Avoid multiple freeze-thaw cycles. Instead, make a series of aliquots from the stock peptide library set.