Neurons are dynamic entities, sensing and responding to their immediate environment. For this reason, understanding protein interactions and signaling mechanisms is a focus of neuroscience research and a key to understanding normal and disease processes. The chance to discover the inner workings of the brain and its components requires a powerful investigative tool. Duolink® Proximity Ligation Assay (PLA) technology allows scientists to visualize those protein interactions, locations and quantities by amplifying signals corresponding to single protein events. This protein detection technology lets researchers visualize protein functions under true biological conditions without overexpression or genetic manipulation, providing a significant resource in advancing neuroscience.
Figure 1. Duolink® Fluorescence Detection P-MAPK p44/p42 in Mouse Brain
Figure 2.Duolink® Brightfield Detection P-MAPK p44/p42 in Mouse Brain