Supplied by Roar Biomedical, Inc.
Catalog Number: MAK110
Storage Temperature 2-8 °C
The kit is sufficient for 100 assays in 200 µL total assay volume.
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Briefly centrifuge vials before opening.
The kit is shipped on wet ice. Storage at 2–8 °C, protected from light, is recommended. DO NOT FREEZE.
Components are stable for 1 year, if stored properly.
All samples and standards should be run in duplicate.
Purified MTP must be used with CP-346086. In this procedure, MTP was purified from rat liver microsomes.
Note: Chill buffers and rotor
3. Add 1 µL of each CP-346086 dilution to separate, mixing well by pipetting. Add 5 µL of the purified MTP, again mixing well by pipetting. Generating final inhibitor concentrations of 55, 11.1, 5.5, 1.11, and 0.111 nM.
4. To a Sample Blank, add 5 µL of MTP Assay Buffer and 1 µL of DMSO, in place of sample and
5. Seal plate and incubate for 60 minutes at 37 °C
6. Measure the fluorescence of validation samples using a fluorometer (λex = 465/λem = 535 nm).
Figure 1.MTP Activity in Rat Liver Microsomes
Figure 2.MTP Inhibition with CP-346086