HomeSmall Molecule HPLCLC/MS/MS Analysis of Epinephrine, Metanephrine and Metabolites on Ascentis® Express OH5
LC/MS/MS Analysis of Epinephrine, Metanephrine and Metabolites on Ascentis® Express OH5
Conditions | |
|---|---|
| column | Ascentis® Express OH5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53757-U) |
| column temp. | 35 °C |
| mobile phase | [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile; (15:85, A:B) |
| flow rate | 0.3 mL/min |
| pressure | 1189 psi (82 bar) |
| sample | 300 ng/mL each in acetonitrile |
| injection | 1 µL |
| detector | MS ESI(+), 100-1000 m/z |
Description | |
|---|---|
| Analysis Note | HILIC mode on Ascentis® Express OH5 provided rapid separation of neurotransmitters in highly MS-compatible mobile phases. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification. |
| Categories | Analytical Chromatography, Clinical, amines, Amines |
| Featured Industry | Clinical |
| Legal Information | Ascentis is a registered trademark of Sigma-Aldrich Co. LLC |
| suitability | application for HPLC |
Materials
Loading
Sign In To Continue
To continue reading please sign in or create an account.
Don't Have An Account?