UHPLC (Isocratic) Analysis of 14 Cannabinoids on Ascentis® Express C18
Materials
analytical column
accessory
standard
Cannabidiol solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Cannabinol solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®(−)-trans-Δ9-THC solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®(−)-Δ8-THC solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Cannabidivarin solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Cannabigerol solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Cannabigerolic acid solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Cannabichromene solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Cannabidiolic acid solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Tetrahydrocannabivarin (THCV) solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Delta9-Tetrahydrocannabinolic acid A solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Cannabidivarinic Acid (CBDVA) solution
1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®Cannabichromenic Acid (CBCA) solution
1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®Tetrahydrocannabivarinic acid (THCVA) solution
1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®CONDITIONS
column
Ascentis Express C18, 15 cm x 2.1 mm, 2.7 μm particles (53825-U)
mobile phase
[A] 0.1% formic acid in HPLC water; [B] 0.1% formic acid in acetonitrile; (30:70, A:B)
flow rate
0.6 mL/min
pressure
6390 psi (440 bar)
column temp.
20 °C
detector
UV, 230 nm
injection
2 μL
sample
25 μg/mL each compound in acetonitrile
Description
Analysis Note
Cannabinoids are a group of active compounds found in cannabis. They reportedly provide relief to an assortment of symptoms in the treatment of pain, nausea, anxiety, mood disorders, and inflammatory diseases. Cannabinoids imitate naturally occurring compounds and interact with two specific types of receptors: CB1 and CB2, located in the nervous system and immune system, respectively. Shown here is an isocratic separation of fourteen cannabinoids in less than twelve minutes on an Ascentis® Express C18 column. Cerilliant cannabinoid CRMs provide reliable identification and quantification. These standards are used in testing methods by GC/MS, LC/MS, or HPLC for applications in clinical toxicology, testing of cannabis potency or impurity profiling by growers, pharmaceutical research, forensic analysis, and urine drug testing.
Legal Information
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