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Physiological Status and Community Composition of Microbial Mats of the Ebro Delta, Spain, by Signature Lipid Biomarkers.

Microbial ecology (2000-05-03)
Navarrete, Peacock, Macnaughton, Urmeneta, Mas-Castellà, White, Guerrero
ABSTRACT

Physiological status of microbial mats of the Ebro Delta (Tarragona, Spain) based on the extraction of lipids considered "signature lipid biomarkers" (SLB) from the cell membranes and walls of microorganisms has been analyzed. Data from a day-night cycle show significant differences in viable cells countings (PLFA cells counts) ranging from 1.5 x 10(10) to 5.0 x 10(10) cells g(-1) of sediment. Minimum values were observed at 18:00 and 6:00, when physicochemical conditions change drastically. The diversity of the microbial community was assessed by GC/MS analysis of phospholipid fatty acids (PLFA). The ratio of PLFA, representative of Gram-negative bacteria, comprises 47.8% of the total PLFA of the microbial mat community. The remaining PLFA was representative of Gram-positive (10.0%), anaerobic (5.7%), and eukaryotic microorganisms (5.7%), and other common lipids. Two different approaches were used as a comparative study to assess the physiological status of the microbial mats. Two parameters (cyclopropane fatty acids/omega7c monoenoic fatty acids, and measurement of the trans/cis monoenoic PLFA ratio) showed a minimum at midnight, suggesting the highest microbial activity. Higher values were observed at 18:00 and 6:00, coinciding with lower PLFA cell counts. </hea

MATERIALS
Product Number
Brand
Product Description

Supelco
SLB®-1ms Capillary GC Column, L × I.D. 10 m × 0.10 mm, df 0.40 μm
Supelco
SLB®-1ms Capillary GC Column, L × I.D. 10 m × 0.10 mm, df 0.10 μm
Supelco
SLB®-1ms Capillary GC Column, L × I.D. 30 m × 0.32 mm, df 0.10 μm
Supelco
SLB®-1ms Capillary GC Column, L × I.D. 60 m × 0.32 mm, df 0.25 μm
Supelco
SLB®-1ms Capillary GC Column, L × I.D. 30 m × 0.32 mm, df 0.25 μm
Supelco
SLB®-1ms Capillary GC Column, L × I.D. 20 m × 0.18 mm, df 0.18 μm
Supelco
SLB®-1ms Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.10 μm
Supelco
SLB®-1ms Capillary GC Column, L × I.D. 60 m × 0.25 mm, df 0.25 μm
Supelco
SLB®-1ms Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.25 μm
Supelco
SLB®-1ms Capillary GC Column, L × I.D. 15 m × 0.25 mm, df 0.25 μm
Supelco
SLB®-1ms Capillary GC Column, L × I.D. 15 m × 0.32 mm, df 0.25 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 1.00 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 15 m × 0.25 mm, df 0.25 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.50 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.32 mm, df 0.25 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.32 mm, df 0.50 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.53 mm, df 1.00 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 10 m × 0.10 mm, df 0.10 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 15 m × 0.10 mm, df 0.10 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 20 m × 0.18 mm, df 0.18 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.25 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 60 m × 0.25 mm, df 0.25 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.10 μm