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Efficient label-free chemiluminescent immunosensor based on dual functional cupric oxide nanorods as peroxidase mimics.

Biosensors & bioelectronics (2017-09-25)
Juan Li, Yue Cao, Samuel S Hinman, Kristy S McKeating, Yiwen Guan, Xiaoya Hu, Quan Cheng, Zhanjun Yang
ABSTRACT

Dual-functional cupric oxide nanorods (CuONRs) as peroxidase mimics are proposed for the development of a flow-through, label-free chemiluminescent (CL) immunosensor. Forming the basis of this cost-efficient, label-free immunoassay, CuONRs, synthesized using a simple hydrothermal method, were deposited onto epoxy-activated standard glass slides, followed by immobilization of biotinylated capture antibodies through a streptavidin bridge. The CuONRs possess excellent catalytic activity, along with high stability as a solid support. Antigens could then be introduced to the sensing system, forming large immunocomplexes that prevent CL substrate access to the surface, thereby reducing the CL signal in a concentration dependent fashion. Using carcinoembryonic antigen (CEA) as a model analyte, the proposed label-free immunosensor was able to rapidly determine CEA with a wide linear range of 0.1-60ngmL

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(3-Glycidyloxypropyl)trimethoxysilane, ≥98%
Sigma-Aldrich
2-Chloroethyl acrylate, contains >100 ppm MEHQ as inhibitor, 97%