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A convenient UHPLC-MS/MS method for routine monitoring of plasma and brain levels of nicotine and cotinine as a tool to validate newly developed preclinical smoking model in mouse.

BMC neuroscience (2017-10-13)
Mohammad A Kaisar, Raja Reddy Kallem, Ravi K Sajja, Ali Ehsan Sifat, Luca Cucullo
ABSTRACT

A sensitive, rapid and selective UHPLC-MS/MS method has been developed and validated for the quantification of Nicotine (NT) and Cotinine (CN) using Continine-d A linear response function was established for the range of concentrations 3-200 (r > 0.995) for NT and 3-600 ng/mL (r > 0.995) for CN. The intra- and inter-day precision values met the acceptance criteria. NT and CN are stable in the battery of stability studies viz., stock solution, bench-top and auto-sampler. This method was successfully utilized to validate a newly developed preclinical smoking model in mice.

MATERIALS
Product Number
Brand
Product Description

Supelco
(−)-Cotinine solution, drug standard, 1.0 mg/mL in methanol