Solid-film sampling method for the determination of protein secondary structure by Fourier transform infrared spectroscopy.

Analytical and bioanalytical chemistry (2017-05-21)

Junting Zhang, Xiaoning Zhang, Fan Zhang, Shaoning Yu

ABSTRACT

Fourier transform infrared (FTIR) spectroscopy is one of the widely used vibrational spectroscopic methods in protein structural analysis. The protein solution sample loaded in demountable CaF

MATERIALS

Product Number

Brand

Product Description

M0630

Sigma-Aldrich

Myoglobin from equine skeletal muscle, 95-100%, essentially salt-free, lyophilized powder

C7752

Sigma-Aldrich

Cytochrome c from equine heart, ≥95% based on Mol. Wt. 12,384 basis

H4632

Sigma-Aldrich

Hemoglobin equine, lyophilized powder

T1426

Sigma-Aldrich

Trypsin from bovine pancreas, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

A7030

Sigma-Aldrich

Bovine Serum Albumin, heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%

A9731

Sigma-Aldrich

Albumin human, recombinant, expressed in rice, lyophilized powder, suitable for cell culture, low endotoxin, ≥96% (SDS-PAGE)