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  • B cells from patients with multiple sclerosis induce cell death via apoptosis in neurons in vitro.

B cells from patients with multiple sclerosis induce cell death via apoptosis in neurons in vitro.

Journal of neuroimmunology (2017-06-12)
Robert P Lisak, Liljana Nedelkoska, Joyce A Benjamins, Dana Schalk, Beverly Bealmear, Hanane Touil, Rui Li, Gillian Muirhead, Amit Bar-Or
ABSTRACT

B cells mediate multiple sclerosis (MS) pathogenesis by mechanisms unrelated to immunoglobulin (Ig). We reported that supernatants (Sup) from cultured B cells from blood of relapsing remitting MS (RRMS) patients, but not normal controls (NC), were cytotoxic to rat oligodendrocytes (OL). We now show that RRMS blood B cells, not stimulated in vitro, secrete factor/s toxic to rat and human neurons. Cytotoxicity is independent of Ig and multiple cytokines, not complement-mediated, and involves apoptosis. The factor/s have an apparent mw of >300kDa. B cells could contribute to damage within the central nervous system by secreting molecules toxic to OL and neurons.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Poly-D-lysine hydrobromide, mol wt >300,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture